LSBio
Human Osteonectin / SPARC ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Human Osteonectin / SPARC ELISA Kit (Sandwich ELISA)

ELISA KitLSBio Guarantee
Reactivity:Human
Detection MethodColorimetric
Format:96-Well Strip Plate
See all Osteonectin / SPARC antibodies →
$597.00each
Catalog Number: LS-F12653-1

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Overview

Description
Osteonectin / SPARC ELISA Kit LS-F12653 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Human Osteonectin / SPARC in Plasma, Serum. It is a Sandwich assay which can detect Osteonectin / SPARC down to 3.12 ng/ml. For Research Use Only
Reactivity
Hu
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits
Alternative Names
Basement-membrane protein 40 | BM-40 | Cysteine-rich protein | ON | Osteonectin | SPARC

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Detection Range
3.12 - 200 ng/ml
Intra-Assay CV%
<10%
Inter-Assay CV%
<12%
Compatible Sample Types
Plasma, Serum
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Standard (Lyophilized), Sample Diluent, Assay Diluent A, Assay Diluent B, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Adhesive Plate Sealers, Wash Buffer (25x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual
Overexpression of SPARC correlates with poor prognosis in patients with cervical carcinoma and regulates cancer cell epithelial-mesenchymal transition. Shi D, Jiang K, Fu Y, Fang R, Liu XI, Chen J. Oncology letters. 2016 May;11:3251-3258. PubMed: 27123099 PMC: PMC4841103
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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.