LSBio
Human NPHS1 / Nephrin ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Human NPHS1 / Nephrin ELISA Kit (Sandwich ELISA)

Reactivity:Human
Detection MethodColorimetric
Format:96-Well Microplate
See all NPHS1 / Nephrin antibodies →
$787.00each
Catalog Number: LS-F21185-1

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Overview

Description
NPHS1 / Nephrin ELISA Kit LS-F21185 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Human NPHS1 / Nephrin in Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates. It is a Sandwich assay which can detect NPHS1 / Nephrin down For Research Use Only
Reactivity
Hu
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits
Alternative Names
Nephrin | NPHN | NPHS1 | CNF

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
0.09 ng/ml
Detection Range
0.16 - 10 ng/ml
Intra-Assay CV%
<5.35%
Inter-Assay CV%
<8.32%
Compatible Sample Types
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Microplate
Kit Components
Coated 96-well Strip Plate, Standard (Lyophilized), Sample Diluent, Biotinylated Detection Antibody (100x), Biotinylated Detection Antibody Diluent, HRP Conjugate (100x), HRP Conjugate Diluent, Wash Buffer (25x), TMB Substrate, Stop Solution, Adhesive Plate Sealers

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual
Urinary Nephrin as a Biomarker of Glomerular Maturation and Injury Is Associated with Acute Kidney Injury and Mortality in Critically Ill Neonates. Jiao Chen, Gen Li, Sanfeng Wang, Xiaohan Hu, Yunqing Sun, Xiaomei Dai, Zhenjiang Bai, Jian Pan, Xiaozhong Li, Jian Wang, Yanhong Li. Neonatology. 2019 April;116:58-66. PubMed: 30982033

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