LSBio
Human NF-L / NEFL ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Human NF-L / NEFL ELISA Kit (Sandwich ELISA)

ELISA KitLSBio Guarantee
Reactivity:Human
Detection MethodColorimetric
Format:96-Well Strip Plate
See all NF-L / NEFL antibodies →
$745.00each
Catalog Number: LS-F6701-1

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Overview

Description
NF-L / NEFL ELISA Kit LS-F6701 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Human NF-L / NEFL in Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates. It is a Sandwich assay which can detect NF-L / NEFL down to 15.6 pg/m For Research Use Only
Reactivity
Hu
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits
Alternative Names
68 kDa neurofilament protein | CMT1F | CMT2E | NEFL | NF68 | NF-L | NFL | Neurofilament subunit NF-L | PPP1R110 | Neurofilament light polypeptide | CMTDIG

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
5.7 pg/ml
Detection Range
15.6 - 1000 pg/ml
Intra-Assay CV%
<10%
Inter-Assay CV%
<12%
Compatible Sample Types
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Adhesive Plate Sealers, Standard (Lyophilized), Sample Diluent, Detection Reagent A (Green), Detection Reagent B (Red), Assay Diluent A, Assay Diluent B, TMB Substrate, Stop Solution, Wash Buffer (30x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual

Microbiome-Derived Lipopolysaccharide (LPS) Selectively Inhibits Neurofilament Light Chain (NF-L) Gene Expression in Human Neuronal-Glial (HNG) Cells in Primary Culture. Lukiw WJ, Cong L, Jaber V, Zhao Y. Frontiers in neuroscience. 2018 December;12:896.

PubMed: 30568571 · PMC: PMC6289986

Facility-based and home-based multidomain interventions including cognitive training, exercise, diet, vascular risk management, and motivation for older adults: a randomized controlled feasibility trial10<sup> Department of Neurology, Hanyang University College of Medicine, Seoul 04763, Republic of Korea12<sup> Lab for Neurodegenerative Dementia, Department of Anatomy, Ajou University School of Medicine, Suwon 16499, Republic of Korea14<sup> Department of Biomedical Systems Engineering, Korea Po. So Young Moon, Chang Hyung Hong, Jee Hyang Jeong, Yoo Kyoung Park, Hae Ri Na, Hong-Sun Song, Byeong C Kim, Kyung Won Park, Hee Kyung Park, Muncheong Choi, Sun Min Lee, Buong-O Chun, Seong-Ho Koh , Sun Ah Park , Hyun-Hee Park, Jeong-Hwa Jin, Eun-Hye Lee, Sue Min Kim , Song Mi Han , Jun Seok Kim, Jungsoon Ha, Seong Hye Choi. Aging. 2021 Jun;13:15898-15916.

PubMed: 34148030 · PMC: PMC8266338

Phase I Trial of Intra-arterial Administration of Autologous Bone Marrow-Derived Mesenchymal Stem Cells in Patients with Multiple System Atrophy. Seok Jong Chung , Tae Yong Lee, Yang Hyun Lee, KyoungWon Baik, Jin Ho Jung , Han Soo Yoo, Chang Jae Shim, Hyojin Eom, Ji-Yeon Hong, Dong Joon Kim, Young H Sohn, Phil Hyu Lee. Stem cells international. 2021 Oct;2021:9886877.

PubMed: 34712335 · PMC: PMC8548132

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