LSBio
Human COL10A1 / Collagen X ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Human COL10A1 / Collagen X ELISA Kit (Sandwich ELISA)

Reactivity:Human
Detection MethodColorimetric
Format:96-Well Strip Plate
See all COL10A1 / Collagen X antibodies →
$683.00each
Catalog Number: LS-F13131-1

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Overview

Description
COL10A1 / Collagen X ELISA Kit LS-F13131 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Human COL10A1 / Collagen X in Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates. It is a Sandwich assay which can detect COL10A1 / Collagen X dow For Research Use Only
Reactivity
Hu
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits
Alternative Names
COL10A1 | Collagen alpha-1(X) chain | Collagen, type X, alpha 1

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
0.098 ng/ml
Detection Range
0.156 - 10 ng/ml
Intra-Assay CV%
<6.2%
Inter-Assay CV%
<11.2%
Compatible Sample Types
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Standard (Lyophilized), Sample Diluent, Assay Diluent A, Assay Diluent B, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Adhesive Plate Sealers, Wash Buffer (25x)

Storage & Handling

Storage Conditions
Short term: -20°C; Long term: -20°C.

Documentation

Manuals
Manual

MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN. Chen L, Li Q, Wang J, Jin S, Zheng H, Lin J, He F, Zhang H, Ma S, Mei J, Yu J. Journal of cellular and molecular medicine. 2017 December;21:3347-3359.

PubMed: 28609022 · PMC: PMC5706578


Juvenile idiopathic arthritis fibroblast-like synoviocytes influence chondrocytes to alter BMP antagonist expression demonstrating an interaction between the two prominent cell types involved in endochondral bone formation. Megan M Simonds, Amanda R Schlefman, Suzanne M McCahan, Kathleen E Sullivan, Carlos D Rose, AnneMarie C Brescia. Pediatric rheumatology online journal. 2020 November;18:89.

PubMed: 33198759 · PMC: PMC7670793


The culture microenvironment of juvenile idiopathic arthritis synovial fibroblasts is favorable for endochondral bone formation through BMP4 and repressed by chondrocytes. Megan M Simonds, Amanda R Schlefman, Suzanne M McCahan, Kathleen E Sullivan, Carlos D Rose, Anne Marie C Brescia. Pediatric rheumatology online journal. 2021 May;19:72.

PubMed: 33980237 · PMC: PMC8117630


Methotrexate inhibits BMP4 and abrogates the hypertrophic chondrocyte phenotype of synovial fibroblasts in juvenile idiopathic arthritis. Megan M Simonds, Samuel T Freer, Anne Marie C Brescia. Pediatric rheumatology online journal. 2024 January;22:6.

PubMed: 38166938 · PMC: PMC10763212

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