LSBio
Estradiol ELISA Kit (Competitive EIA)

Competition ELISA Platform Overview

Estradiol ELISA Kit (Competitive EIA)

Detection MethodColorimetric
Format:96-Well Strip Plate
See all Estradiol antibodies →
$498.00each
Catalog Number: LS-F5297-1

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Overview

Description
Estradiol ELISA Kit LS-F5297 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of All species Merged into BiotargetID 235: Estradiol in Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates. It is a Competitive EIA assay.
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Competitive Enzyme-Linked Immunosorbent Assay
Sensitivity
4.93 pg/ml
Detection Range
12.35 - 1000 pg/ml
Intra-Assay CV%
<10%
Inter-Assay CV%
<12%
Compatible Sample Types
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Adhesive Plate Sealers, Standard (Lyophilized), Sample Diluent, Detection Reagent A (Green), Detection Reagent B (Red), Assay Diluent A, Assay Diluent B, TMB Substrate, Stop Solution, Wash Buffer (30x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual
Alterations of estrous cycle, 3ß hydroxysteroid dehydrogenase activity and progesterone synthesis in female rats after exposure to hypobaric hypoxia. Snigdha Shaw, Utkarsha Kumar, Gopinath Bhaumik, M Prasanna Kumar Reddy, Bhuvnesh Kumar, Dishari Ghosh. Scientific reports. 2020 Feb;10:3458. PubMed: 32103034 PMC: PMC7044287

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.