LSBio
Bovine TNF Alpha ELISA Kit (Sandwich ELISA)

Sandwich ELISA Platform Overview

Bovine TNF Alpha ELISA Kit (Sandwich ELISA)

ELISA KitLSBio Guarantee
Reactivity:Bovine
Detection MethodColorimetric
Format:96-Well Strip Plate
See all TNF Alpha antibodies →
$823.00each
Catalog Number: LS-F5014-1

For bulk orders or custom formulations:

Request a Quote

Overview

Description
TNF Alpha ELISA Kit LS-F5014 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Bovine TNF Alpha in Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates. It is a Sandwich assay which can detect TNF Alpha down to 7.8 pg/ml. For Research Use Only
Reactivity
Bov
Intended Use
Research Use Only
Category
ELISA Kits > Traditional ELISA Kits
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2

Assay Performance

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Sandwich Enzyme-Linked Immunosorbent Assay
Sensitivity
7.8 pg/ml
Detection Range
7.8 - 500 pg/ml
Intra-Assay CV%
<10%
Inter-Assay CV%
<12%
Compatible Sample Types
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
450 nm
About This Kit
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Format

Format
96-Well Strip Plate
Kit Components
Coated 96-well Strip Plate, Adhesive Plate Sealers, Standard (Lyophilized), Sample Diluent, Detection Reagent A (Green), Detection Reagent B (Red), Assay Diluent A, Assay Diluent B, TMB Substrate, Stop Solution, Wash Buffer (30x)

Storage & Handling

Storage Conditions
Short term: 4°C; Long term: see manual.

Documentation

Manuals
Manual

Effects of arginase inhibition via jugular infusion of N?-hydroxy-nor-l-arginine on metabolic and immune indices in lactating dairy cows. L Y Ding, Y F Wang, Y Z Shen, G Zhou, X Zhang, M Z Wang, J J Loor, J Zhang. Journal of dairy science. 2019 Apr;102:3310-3320.

PubMed: 30738683

Histamine promotes adhesion of neutrophils by inhibition of autophagy in dairy cows with subacute ruminal acidosis. Kexin Wang, Zhenai Sun, Yunfei Li, Mingchao Liu, Juan J Loor, Qianming Jiang, Guowen Liu, Zhe Wang, Yuxiang Song, Xinwei Li. Journal of dairy science. 2022 September;105:7600-7614.

PubMed: 35940921

Free fatty acids promote degranulation of azurophil granules in neutrophils by inducing production of NADPH oxidase-derived reactive oxygen species in cows with subclinical ketosis. Yuxiang Song, Shang Jiang, Congyi Li, Juan J Loor, Qianming Jiang, Yuchen Yang, Xiancheng Feng, Siyuan Liu, Jiyuan He, Kexin Wang, Yunfei Li, Cai Zhang, Xiliang Du, Zhe Wang, Xinwei Li, Guowen Liu. Journal of dairy science. 2022 March;105:2473-2486.

PubMed: 34998570

Cellular and mitochondrial adaptation mechanisms in the colon of lactating dairy cows during hyperthermia. Mehdi Eslamizad, Dirk Albrecht, Björn Kuhla, Franziska Koch. Journal of dairy science. 2023 December.

PubMed: 38056565

Submit a Publication

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.