AMPK Phosphorylation Assay Kit (Fluorometric)
Catalog Number: LS-K268-100
100 tests / $694.00
Assay Format Biochemical Assay
Format: Kit
NFkB Phosphorylation Assay Kit (Fluorometric)
Assay KitLSBio Guarantee
| Assay Format | Biochemical Assay |
| Format: | Kit |
$694.00each
Catalog Number: LS-K271-100
Catalog Number: LS-K271
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- Description
- Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-alpha. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcription. This cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.
- Target
- NFkB Phosphorylation
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Fluorescent / Fluorometric
- Assay Type
- Quantitative
- Assay Format
- Biochemical Assay
- Compatible Sample Types
- Cells, Tissue Homogenate
Format
- Format
- Kit
- Kit Components
- 10 Wash Buffer, Blocking Buffer, Protein Stain, HRP Substrate, pNF kkk kB-Ab1, HRP-Ab2 10 µL
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document
