Malate Dehydrogenase (MDH) Assay Kit (Colorimetric)

Assay Format	Enzyme Activity Assay
Format:	Kit

$577.00each

Catalog Number: LS-K273-100

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Description: Malate dehydrogenase (MDH) (EC 1.1.1.37) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 found in the cytoplasm and plays a key part in the malate-aspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer's disease. This non-radioactive, colorimetric MDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Target: Malate Dehydrogenase (MDH)
Category: Assay Kits > All Assay Kits

Detection Method: Colorimetric
Assay Type: Quantitative
Assay Format: Enzyme Activity Assay
Compatible Sample Types: Cell Culture Supernatant, Cells, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric): 565 nm