LSBio
Kinase Assay Kit (Fluorometric)

Kinase Assay Kit (Fluorometric)

Assay FormatEnzyme Activity Assay
Format:Kit
See all Kinase antibodies →
$388.00each
Catalog Number: LS-K294-400

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Overview

Description
Kinases , also known as phosphotransferases, constitute a large family of enzymes that transfer phosphate groups from the high-energy donor molecule ATP, to their specific substrates. Kinases are known to regulate the majority of cellular processes. The largest group of this family is the protein kinases. So far, 518 different kinases have been identified in humans and up to 30% of human proteins are modified by these kinases. The enormous diversity and their key role in cellular signaling make them ideal targets for drug developments. This Kinase Assay Kit provides a simple and rapid Method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorimetric (530nm/590nm) assay Method.
Target
Kinase
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Fluorescent / Fluorometric
Assay Type
Quantitative
Assay Format
Enzyme Activity Assay

Format

Format
Kit
Kit Components
Reagent A, Reagent B, Assay Buffer, standard 3 mM ADP

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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