
Invertase Assay Kit (Colorimetric/Fluorometric)
| Assay Format | Enzyme Activity Assay |
| Format: | Kit |
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- Description
- Invertase (b-fructofuranosidase, EC 3.2.1.26) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrase (EC 3.2.1.48) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural and food (confectionery) industry. This Invertase Assay Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric Method (lambda ex/em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.
- Target
- Invertase
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Colorimetric, Fluorescent / Fluorometric
- Assay Type
- Quantitative
- Assay Format
- Enzyme Activity Assay
- Compatible Sample Types
- Other Biological Sample
- Detection Wavelength (Colorimetric)
- 570 nm
Format
- Format
- Kit
- Kit Components
- 10x Reaction Buffer (pH 4.5), 10x Sucrose, Assay Buffer, Enzyme Mix, Dye Reagent, Glucose Standard (300 mg\/dL)
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document




















