LSBio
Invertase Assay Kit (Colorimetric/Fluorometric)

Invertase Assay Kit (Colorimetric/Fluorometric)

Assay FormatEnzyme Activity Assay
Format:Kit
See all Invertase antibodies →
$597.00each
Catalog Number: LS-K210-100

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Overview

Description
Invertase (b-fructofuranosidase, EC 3.2.1.26) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrase (EC 3.2.1.48) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural and food (confectionery) industry. This Invertase Assay Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric Method (lambda ex/em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.
Target
Invertase
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Colorimetric, Fluorescent / Fluorometric
Assay Type
Quantitative
Assay Format
Enzyme Activity Assay
Compatible Sample Types
Other Biological Sample
Detection Wavelength (Colorimetric)
570 nm

Format

Format
Kit
Kit Components
10x Reaction Buffer (pH 4.5), 10x Sucrose, Assay Buffer, Enzyme Mix, Dye Reagent, Glucose Standard (300 mg\/dL)

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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