LSBio
Glucose dehydrogenase (GDH) Assay Kit (Colorimetric)

Glucose dehydrogenase (GDH) Assay Kit (Colorimetric)

Assay FormatEnzyme Activity Assay
Format:Kit
See all Glucose dehydrogenase (GDH) antibodies →
$490.00each
Catalog Number: LS-K274-100

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Overview

Description
GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway. This non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Target
Glucose dehydrogenase (GDH)
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Enzyme Activity Assay
Compatible Sample Types
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
565 nm

Format

Format
Kit
Kit Components
Assay Buffer, Diaphorase, NAD \/MTT, Calibrator, Substrate

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
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