G6PDH Assay Kit (Colorimetric)
Assay KitLSBio Guarantee
| Assay Format | Biochemical Assay |
| Format: | Kit |
$541.00each
Catalog Number: LS-K280-100
Catalog Number: LS-K280
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- Description
- GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. This non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
- Target
- G6PDH
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Colorimetric
- Assay Type
- Quantitative
- Assay Format
- Biochemical Assay
- Compatible Sample Types
- Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
- Detection Wavelength (Colorimetric)
- 460 nm
Format
- Format
- Kit
- Kit Components
- Assay Buffer, Diaphorase, NAD P\/MTT, Calibrator, Substrate
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document
