LSBio
Free Fatty Acid Assay Kit (Colorimetric/Fluorometric)

Free Fatty Acid Assay Kit (Colorimetric/Fluorometric)

Assay FormatBiochemical Assay
Format:Kit
See all Free Fatty Acid antibodies →
$577.00each
Catalog Number: LS-K170-100

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Overview

Description
Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome. This Method provides a simple, one-step and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.
Target
Free Fatty Acid
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Colorimetric, Fluorescent / Fluorometric
Assay Type
Quantitative
Assay Format
Biochemical Assay
Compatible Sample Types
Cell Culture Supernatant, Food Sample, Other Biological Sample, Plasma, Saliva, Serum, Urine
Detection Wavelength (Colorimetric)
570 nm

Format

Format
Kit
Kit Components
Assay Buffer, Dye Reagent, Enzyme A: Dried, CoSubstrate, Standard 1 mM Palmitic Acid, Enzyme B

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.