
Choline Assay Kit (Colorimetric/Fluorometric)
| Assay Format | Biochemical Assay |
| Format: | Kit |
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- Description
- CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. This Method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to Betaine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.
- Target
- Choline
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Colorimetric, Fluorescent / Fluorometric
- Assay Type
- Quantitative
- Assay Format
- Biochemical Assay
- Compatible Sample Types
- Cells, Milk, Plasma, Saliva, Serum, Tissue Homogenate, Urine
- Detection Wavelength (Colorimetric)
- 570 nm
Format
- Format
- Kit
- Kit Components
- Assay Buffer, Enzyme Mix: Dried Dye Reagent, standard 2 mM Choline, Dye Reagent
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document




















