LSBio
Alpha-Mannosidase (AMA) Assay Kit (Colorimetric)

Alpha-Mannosidase (AMA) Assay Kit (Colorimetric)

Assay FormatCell Viability / Cytotoxicity Assay
Format:Kit
See all Alpha-Mannosidase (AMA) antibodies →
$541.00each
Catalog Number: LS-K195-100

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Overview

Description
Alpha-MANNOSIDASE (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. Alpha-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes Alpha-mannosidosis and leads to deterioration of the central nervous system in children. This non-radioactive, colorimetric AMA assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Target
Alpha-Mannosidase (AMA)
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Cell Viability / Cytotoxicity Assay
Compatible Sample Types
Other Biological Sample
Detection Wavelength (Colorimetric)
405 nm

Format

Format
Kit
Kit Components
Substrate, Stop Reagent, Standard 12.5 mM Nitrophenol

Storage & Handling

Storage Conditions
Store at 4°C.

Documentation

Documents
Document

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.