LSBio
Adenosine Diphosphate (ADP) Assay Kit (Chemiluminescent)

Adenosine Diphosphate (ADP) Assay Kit (Chemiluminescent)

Assay FormatCell Viability / Cytotoxicity Assay
Format:Kit
See all Adenosine Diphosphate (ADP) antibodies →
$470.00each
Catalog Number: LS-K257-100

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Overview

Description
This ADP Assay Kit provides a rapid Method to measure ADP levels. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
Target
Adenosine Diphosphate (ADP)
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Luminescent / Chemiluminescent
Assay Type
Quantitative
Assay Format
Cell Viability / Cytotoxicity Assay
Compatible Sample Types
Cells

Format

Format
Kit
Kit Components
Assay Buffer, Substrate, Cosubstrate, ATP Enzyme, ADP Enzyme, standard 3 mM ADP

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.