1:100 staining human lymph node tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ZIF268 / EGR1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C800582-100
Catalog Number: LS-C800582
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- EGR1 antibody LS-C800582 is an unconjugated rabbit polyclonal antibody to EGR1 (ZIF268) from human. It is reactive with human, mouse and monkey. Validated for IF, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AT225 | EGR1 | Early growth response 1 | G0S30 | KROX24 | NGFIA | KROX-24 | TIS8 | ZIF-268 | ZIF268 | Zinc finger protein Krox-24 | EGR-1 | NGFI-A | Transcription factor ETR103 | Transcription factor Zif268 | Zinc finger protein 225
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P18146
- NCBI GenBank
- NM_001964 | NP_001955.1
- SwissProt
- P18146
Target
- Target
- Human ZIF268 / EGR1
- Antigen Species
- Human
- Epitope
- EGR1 Antibody detects endogenous levels of EGR1
- Gene Name
- EGR1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human EGR1.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS