Anti-ZIC2 Antibody - Western Blot. Western blot of Affinity Purified anti-ZIC2 antibody shows detection of a band ~55 kD (arrowhead) corresponding to ZIC2 in lysates from (lane 1) mouse brain and (lane 2) rat brain. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-ZIC2 antibody. Signal was detected using standard techniques. The upper band in the mouse brain lysate represents Zic2.

ZIC2 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$579.00each

Catalog Number: LS-C19096-100

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Overview

Description: ZIC2 antibody LS-C19096 is an unconjugated rabbit polyclonal antibody to ZIC2 from mouse. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Application: ELISA, IHC, WB
Reactivity: Hu
Predicted Reactivity: Dog, Mouse, Non-Human Primate, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HPE5 | ZIC2 | Zic family member 2 | Zinc finger protein ZIC 2
Recommended Dilution: ELISA: 1:10000 - 1:50000
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: O95409
NCBI GenBank: NM_007129 | NP_009060.2
SwissProt: O95409

Target

Target: Mouse ZIC2
Antigen Species: Mouse
Epitope: A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, mouse, rat and dog based on 100% homology for the immunogen sequence. Expect cross reactivity with ZIC2 from chicken, zebrafish and frog as the core 16 amino acid residues within the immunogen sequence show very high degrees of homology with ZIC2 from these sources. Cross reactivity with ZIC2 homologues from other sources has not been determined.
Gene Name: ZIC2

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 180-210 of Mouse ZIC-2.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified