Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
YWHAZ / 14-3-3 Zeta Antibody (phospho-Ser58)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$341.00each
Catalog Number: LS-C291151-50
Catalog Number: LS-C291151
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- Description
- 14-3-3 Zeta antibody LS-C291151 is an unconjugated rabbit polyclonal antibody to 14-3-3 Zeta (YWHAZ) (pSer58) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 14-3-3 protein zeta/delta | 14-3-3-zeta | 14-3-3 delta | 14-3-3 protein zeta | 14-3-3 zeta | Phospholipase A2 | YWHAD | KCIP-1 | YWHAZ
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P63104
- NCBI GenBank
- NM_003406 | NP_003397.1
- SwissProt
- P63104
Target
- Target
- Human YWHAZ / 14-3-3 Zeta
- Antigen Species
- Human
- Epitope
- 14-3-3 zeta (Phospho-Ser58) Antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Ser58.
- Gene Name
- YWHAZ
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. (AA Range: 24-73)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS|150 mM NaCl