Immunofluorescence staining of YBX1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human YBX1 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.
YBX1 / YB1 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815088-100
Catalog Number: LS-C815088
For bulk orders or custom formulations:
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- Description
- YB1 antibody LS-C815088 is an unconjugated rabbit polyclonal antibody to human YB1 (YBX1). Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CBF-A | DBPB | CSDB | DNA-binding protein B | MDR-NF1 | NSEP-1 | NSEP1 | Y box binding protein 1 | Y-box transcription factor | Y-box-binding protein 1 | YB-1 | YBX1 | BP-8 | CSDA2 | EFI-A | Enhancer factor I subunit A | YB1
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P67809
- NCBI GenBank
- NM_004559 | NP_004550.2
- SwissProt
- P67809
Target
- Target
- Human YBX1 / YB1
- Antigen Species
- Human
- Epitope
- Human YB-1/YBX1
- Gene Name
- YBX1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the center region of the Human YBX1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS