HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WWTR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WWTR1.
WWTR1 / TAZ Antibody (clone OTI1H9)
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
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- Description
- TAZ antibody LS-C173295 is an unconjugated mouse monoclonal antibody to human TAZ (WWTR1). Validated for IHC and WB. Cited in 5 publications.
- Application
- IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- WWTR1
- Recommended Dilution: IHC-P
- 1:150
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- Q9GZV5
- NCBI GenBank
- NM_015472 | NP_056287.1
- SwissProt
- Q9GZV5
Target
- Target
- Human WWTR1 / TAZ
- Antigen Species
- Human
- Epitope
- Human WWTR1
- Gene Name
- WWTR1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI1H9
- Isotype
- IgG2a
Immunogen
- Immunogen
- Full length human recombinant protein of human WWTR1(NP_057287) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
TAZ and YAP are frequently activated oncoproteins in sarcomas. Fullenkamp CA, Hall SL, Jaber OI, Pakalniskis BL, Savage EC, Savage JM, Ofori-Amanfo GK, Lambertz AM, Ivins SD, Stipp CS, Miller BJ, Milhem MM, Tanas MR. Oncotarget. 2016;7:30094-108.
PubMed: 27129148 · PMC: PMC5058666
a3ß1 Integrin Suppresses Prostate Cancer Metastasis via Regulation of the Hippo Pathway. Varzavand A, Hacker W, Ma D, Gibson-Corley K, Hawayek M, Tayh OJ, Brown JA, Henry MD, Stipp CS. Cancer research. 2016 November;76:6577-6587.
PubMed: 27680681 · PMC: PMC5290210
A comprehensive evaluation of Hippo pathway silencing in sarcomas. Merritt NM, Fullenkamp CA, Hall SL, Qian Q, Desai C, Thomason J, Lambertz AM, Dupuy AJ, Darbro B, Tanas MR. Oncotarget. 2018 August;9:31620-31636.
PubMed: 30167083 · PMC: PMC6114978
The effect of YAP expression in tumor cells and tumor stroma on the prognosis of patients with squamous cell carcinoma of the oral cavity floor and oral surface of the tongue. Jolanta Szelachowska, Piotr Donizy, Katarzyna Ratajczak-Wielgomas, Agnieszka Halon, Dominika Zielecka-Debska, Krystian Lichon, Adam Maciejczyk, Ewelina Lata-Wozniak, Aleksandra Piotrowska, Rafal Matkowski. Oncology letters. 2019 Oct;18:3561-3570.
PubMed: 31579068 · PMC: PMC6757271
Prognostic and therapeutic value of the Hippo pathway, RABL6A, and p53-MDM2 axes in sarcomas. Chandni Desai, Jon Thomason, Jordan L Kohlmeyer, Anna C Reisetter, Parmanand Ahirwar, Khadijeh Jahanseir, Mariah Leidinger, Georgina Ofori-Amanfo, Karen Fritchie, Sadanandan E Velu, Patrick Breheny, Dawn E Quelle, Munir R Tanas. Oncotarget. 2021 April;12:740-755.
PubMed: 33889298 · PMC: PMC8057271