Immunofluorescence staining of WDR77 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human WDR77 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
WDR77 / MEP50 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815085-100
Catalog Number: LS-C815085
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- Description
- MEP50 antibody LS-C815085 is an unconjugated rabbit polyclonal antibody to human MEP50 (WDR77). Validated for ICC, IF, IHC and WB.
- Application
- ICC, IF, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Androgen receptor cofactor p44 | HKMT1069 | MEP-50 | Methylosome protein 50 | p44 | MEP50 | Nbla10071 | RP11-552M11.3 | WD repeat domain 77 | WDR77
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q9BQA1
- NCBI GenBank
- NM_024102 | NP_077007.1
- SwissProt
- Q9BQA1
Target
- Target
- Human WDR77 / MEP50
- Antigen Species
- Human
- Epitope
- Human WDR77
- Gene Name
- WDR77
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the center region of the Human WDR77
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS