Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.
WDFY3 / ALFY Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human, Rat |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C773625-100
Catalog Number: LS-C773625
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- ALFY antibody LS-C773625 is an unconjugated rabbit polyclonal antibody to ALFY (WDFY3) from human. It is reactive with human and rat. Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ALFY | Autophagy-linked FYVE protein | KIAA0993 | ZFYVE25 | WDFY3
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q8IZQ1
- SwissProt
- Q8IZQ1
Target
- Target
- Human WDFY3 / ALFY
- Antigen Species
- Human
- Epitope
- Detects ~400 kDa.
- Gene Name
- WDFY3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the N-Terminus of Human WDFY3 (aa. 533-544)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS