Western blot of crude extract of the human carcinoma MCA-2A52 clone. Lane 15 was probed with our antibody to actin, giving an SDS-PAGE molecular weight of 42kDa, and lane 16 with MCA-3G12, our antibody to 14-3-3 eta, which has an SDS-PAGE molecular weight of 28kDa.

Vimentin Antibody (clone 2D1)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Immunocytochemistry, Immunofluorescence, Western Blot
Reactivity:	Bovine, Human, Mouse, Pig, Rat
Format:	Liquid

$384.00each

Catalog Number: LS-C204599-100

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Quantity

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Overview

Description: Vimentin antibody LS-C204599 is an unconjugated mouse monoclonal antibody to Vimentin from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
Application: ICC, IF, WB
Reactivity: Bov, Hu, Ms, Por, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: VIM | Vimentin | vimenin
Recommended Dilution: IF/ICC: 1:1000|0.736111111111111
Recommended Dilution: WB: 1:10000
UniProt Number: P08670
NCBI GenBank: NM_003380 | NP_003371.2
SwissProt: P08670

Target

Target: Human Vimentin
Antigen Species: Human
Epitope: Clone 12D1 is known to react with vimentin from human, cow, pig, mouse, rat and other mammals. Since vimentin is highly conserved, it is likely that the antibody is effective on other species also.
Gene Name: VIM

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 2D1
Isotype: IgG2a
Light Chain: Kappa Light Chain

Immunogen

Immunogen: The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli.
Immunogen Type: Recombinant Immunogen