Detection of Human and Mouse Vimentin by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody: Affinity purified rabbit anti-Vimentin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Vimentin Antibody (aa416-466)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$457.00each

Catalog Number: LS-C288421-100

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Overview

Description: Vimentin antibody LS-C288421 is an unconjugated rabbit polyclonal antibody to Vimentin (aa416-466) from human. It is reactive with human and mouse. Validated for IHC and WB.
Application: IHC, IHC-P, WB
Reactivity: Hu, Ms
Predicted Reactivity: Bovine, Chicken, Hamster, Non-Human Primate, Pig, Rat
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: VIM | Vimentin | vimenin
Recommended Dilution: IHC-P: 1:200 - 1:1000
Recommended Dilution: WB: 1:2000 - 1:10000
UniProt Number: P08670
NCBI GenBank: NM_003380 | NP_003371.2
SwissProt: P08670

Target

Target: Human Vimentin
Antigen Species: Human
Epitope: Region between residue 416 and 466 of human vimentin using the numbering given in entry NP_003371.2 (GeneID 7431).
Gene Name: VIM

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: A portion of GeneID: 7431 corresponding to amino acids between 416 and 466 of UniprotID: P08670
Immunogen Type: Recombinant Protein Fragment