Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-VDCA-1 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-VDCA-1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Mitochondrion Outer Membrane, Cell Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VDCA-1 Antibody. (D) Composite.
VDAC1 / PORIN Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C773571-100
Catalog Number: LS-C773571
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- Description
- PORIN antibody LS-C773571 is an unconjugated rabbit polyclonal antibody to human PORIN (VDAC1). Validated for IF, IHC and WB.
- Application
- IF, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HVDAC1 | Plasmalemmal porin | Porin 31HM | PORIN | PORIN-31-HL | Vdac5 | VDAC | VDAC1 | Mitochondrial porin | Porin 31HL | VDAC-1
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P21796
- NCBI GenBank
- NM_003374 | NP_003365.1
- SwissProt
- P21796
Target
- Target
- Human VDAC1 / PORIN
- Antigen Species
- Human
- Epitope
- Detects ~37 kDa.
- Gene Name
- VDAC1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the N-Terminus of Human VDCA-1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS