P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vinculin (Phospho-Tyr821) antibody.
VCL / Vinculin Antibody (phospho-Tyr821)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general) |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C822493-100
Catalog Number: LS-C822493
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- Description
- Vinculin antibody LS-C822493 is an unconjugated rabbit polyclonal antibody to Vinculin (VCL) (pTyr821) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF and IHC.
- Application
- ELISA, IF, IHC
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CMD1W | CMH15 | Metavinculin | VCL | Vinculin | MVCL
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:100
- UniProt Number
- P18206
- NCBI GenBank
- NM_014000 | NP_054706.1
- SwissProt
- P18206
Target
- Target
- Human VCL / Vinculin
- Antigen Species
- Human
- Epitope
- Vinculin (Phospho-Tyr821) antibody detects endogenous levels of Vinculin only when phosphorylated at tyrosine 821. (Human: Tyr821; Mouse: Tyr821; Rat: Tyr822)
- Gene Name
- VCL
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human Vinculin around the phosphorylation site of tyrosine 821 (S-G-YP-R-I).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl