All lanes: Anti-Vinculin at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: mouse kidney lysate Lane 5: PC-12 whole cell lysate Lane 6: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 117 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
VCL / Vinculin Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C757626-200
Catalog Number: LS-C757626
For bulk orders or custom formulations:
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- Description
- Vinculin antibody LS-C757626 is an unconjugated rabbit polyclonal antibody to Vinculin (VCL) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB.
- Application
- ELISA, FC, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CMD1W | CMH15 | Metavinculin | VCL | Vinculin | MVCL
- Recommended Dilution: FC
- 1:25
- Recommended Dilution: IHC-P
- 1:25
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- P18206
- NCBI GenBank
- NM_014000 | NP_054706.1
- SwissProt
- P18206
Target
- Target
- Human VCL / Vinculin
- Antigen Species
- Human
- Gene Name
- VCL
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 903-937 amino acids from mouse.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 200 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS