LSBio
Uricase Antibody (Liquid, Biotin)

Goat anti Uricase antibody was used to detect purified Uricase under reducing and non-reducing conditions. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE and reduced samples of purified Uricase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Goat anti Uricase and imaged on the BioRad VersaDoc imaging system.

Uricase Antibody (Liquid, Biotin)

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity:Bacteria
Format:Liquid
See all Bacillus Uricase antibodies →
$311.00each
Catalog Number: LS-C744747-25

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Overview

Description
Uricase antibody LS-C744747 is a biotin-conjugated goat polyclonal antibody to bacillus Uricase. Validated for ELISA, IP and WB.
Application
ELISA, IP, WB
Reactivity
Bac
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:1000 - 1:2500
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:1000

Target

Target
Bacillus Uricase
Antigen Species
Bacteria
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species]. Cross reactivity against Uricase from other sources is unknown.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Uricase [Bacillus species].

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.