Immunoblotting of MCA-Ubi-1 on (lane 1) poly-ubiquitin (lys63 linked), (lane 2) pure ubiquitin and lanes 3-5 crude homogenates of adult rat cortex, cerebellum and brain stem.

Ubiquitin Antibody (clone Ubi-1-AP)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$426.00each

Catalog Number: LS-C204828-100

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Overview

Description: Ubiquitin antibody LS-C204828 is an unconjugated mouse monoclonal antibody to human Ubiquitin. Validated for IF, IHC and WB.
Application: IF, IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: IF/ICC: 1:500
Recommended Dilution: IHC: 1:1000

Target

Target: Mammal Ubiquitin
Antigen Species: Mammal (Other)
Epitope: Clone Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that clone Ubi-1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of Alzheimer's disease. The antibody also works on paraffin embedded material. clone Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues. clone Ubi-1 will recognize human, bovine, chicken, Drosophila, and C. elegans in ELISA. clone Ubi-1 also works on Western blots and can be used to study ubiquitinated proteins. It has also been successfully used in competitive ELISA.

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: Ubi-1-AP
Isotype: IgG1
Light Chain: Kappa Light Chain

Immunogen

Immunogen: Purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin. The clone was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. MCA-Ubi-1 was one of several clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically.
Immunogen Type: Native Protein (Purified from Natural Source)