LSBio
Ubiquitin Antibody (clone RGL3R, Biotin)

Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone RGL3R. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG:HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.

Ubiquitin Antibody (clone RGL3R, Biotin)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Recombinant Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Pan-species / Cross-reactive
Format:Liquid
See all All species Ubiquitin antibodies →
$519.00each
Catalog Number: LS-C776806-100

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Overview

Description
Ubiquitin antibody LS-C776806 is a biotin-conjugated rabbit recombinant monoclonal antibody to all species Ubiquitin. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Pan
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Recombinant Antibodies
Recommended Dilution: ELISA
1:1000
Recommended Dilution: WB
1:1000

Target

Target
All species Ubiquitin
Antigen Species
Pan-species / Cross-reactive
Epitope
Detects ubiquitined proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.

Antibody

Host Species
Rabbit
Clonality
Recombinant Monoclonal
Clone
RGL3R
Isotype
IgG

Immunogen

Immunogen
Ubiquitin conjugated lysozyme

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.