The Cell lysates (40ug) and Mouse tissue extract (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tubulin Beta 3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate Lane 3.: Mouse Brain tissue extract
TUBB3 / Tubulin Beta 3 Antibody (clone AT2F5)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C755583-50
Catalog Number: LS-C755583
For bulk orders or custom formulations:
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- Description
- Tubulin Beta 3 antibody LS-C755583 is an unconjugated mouse monoclonal antibody to human Tubulin Beta 3 (TUBB3). Validated for ELISA, Flow, ICC and WB.
- Application
- ELISA, FC, ICC, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CDCBM | Beta III Tubulin | Class III beta-tubulin | TUBB4 | Tubulin, beta 3 | Tubulin beta-III | Tubulin beta-3 chain | Tubulin beta-4 chain | Tubulin, beta 3 class III | CFEOM3A | TUBB3
- UniProt Number
- Q13509
- NCBI GenBank
- NM_006086 | NP_006077.2
- SwissProt
- Q13509
Recommended Dilution
| Application | Dilution |
|---|---|
| IF/ICC | 1:200 |
| WB | 1:1000 |
Target
- Target
- Human TUBB3 / Tubulin Beta 3
- Antigen Species
- Human
- Gene Name
- TUBB3
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AT2F5
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant human TUBB3 (1-450aa) purified from E. coli
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Recommended Storage Buffer
- PBS