Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western blot of Biotin conjugated Rabbit Anti-TSLP primary antibody. Lane 1: Human TSLP. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP Biotin conjugated antibody at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Observed/Predicted size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TSLP Antibody (Lyophilized, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C209279-100
Catalog Number: LS-C209279
For bulk orders or custom formulations:
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- Description
- TSLP antibody LS-C209279 is a biotin-conjugated rabbit polyclonal antibody to human TSLP. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- TSLP | Thymic stromal lymphopoietin
- Recommended Dilution: ELISA
- 1:20000 - 1:50000
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- Q969D9
- NCBI GenBank
- NM_033035 | NP_149024.1
- SwissProt
- Q969D9
Target
- Target
- Human TSLP
- Antigen Species
- Human
- Epitope
- This antibody is specific for human TSLP protein. A BLAST analysis was used to suggest cross-reactivity with TSLP from human sources based on 100% homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. Cross-reactivity with TSLP from other sources has not been determined.
- Gene Name
- TSLP
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
