LSBio
Trypsin Inhibitor Antibody (Lyophilized, HRP)

Western Blot of Rabbit Anti Trypsin Inhibitor antibody. Lane 1: purified Soybean Trypsin Inhibitor Reduced. Lane 2: purified Soybean Trypsin Inhibitor Non-Reduced. Lane 3: purified Streptavidin Reduced. Lane 4: purified Streptavidin Non-Reduced. Load: 1.0 ug per lane. Primary antibody: Biotin conjugated Rabbit anti-trypsin inhibitor antibody and Rabbit anti streptavidin 1:1000 for overnight at 4 degrees C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 25 kDa for Trypsin Inhibitor. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Trypsin Inhibitor Antibody (Lyophilized, HRP)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Plant
Format:Lyophilized
See all Soy Bean Trypsin Inhibitor antibodies →
$564.00each
Catalog Number: LS-C209249-100

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Overview

Description
Trypsin Inhibitor antibody LS-C209249 is an HRP-conjugated rabbit polyclonal antibody to soy bean Trypsin Inhibitor. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Plnt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Soy Bean Trypsin Inhibitor
Antigen Species
Plant
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor (Soy Bean). Cross-reactivity against Trypsin Inhibitor from other tissues and species is unknown.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.