Western blot analysis of extracts of various cell lines, using TRIM5 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.

TRIM5 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C746796-20

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Overview

Description: TRIM5 antibody LS-C746796 is an unconjugated rabbit polyclonal antibody to TRIM5 from human. It is reactive with human and mouse. Validated for WB.
Application: WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: RNF88 | RING finger protein 88 | Tripartite motif containing 5 | TRIM5alpha | Tripartite motif protein TRIM5 | Tripartite motif-containing 5 | TRIM5
Recommended Dilution: WB: 1:500 - 1:2000
NCBI GenBank: NM_033034

Target

Target: Human TRIM5
Antigen Species: Human

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM5 (NP_149083.2). EKLLLFCQEDGKVICWLCERSQEHRGHHTFLTEEVAREYQVKLQAALEMLRQKQQEAEELEADIREEKASWKTQIQYDKTNVLADFEQLRDILDWEESNEL
Immunogen Type: Synthetic Peptide (Linear)