Western Blot of Anti-p53 (ac Lys292) (RABBIT) Antibody. Lane 1: HCT-116 Whole Cell Lysate. Lane 2: A549 WCL. Lane 3: C5B46 Brain Lysate. Lane 4: MCF7 Nuclear Extract Lysate. Lane 5: A431 Nuclear Extract Lysate. Lane 6: HeLa WCL. Lane 7: HeLa Nuclear Extract Lysate. Lane 8: Normal Ms Brain Lysate. Lane 9: Molecular Weight Ladder PreStained Loaded at 10ug. Primary Antibody: Anti-p53 292kAc at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP Predicted: ~43.6 kDa.
TP53 / p53 Antibody (Ac-Lys292)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745410-100
Catalog Number: LS-C745410
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- Description
- P53 antibody LS-C745410 is an unconjugated rabbit polyclonal antibody to human p53 (TP53) (Ac-Lys292). Validated for DB, ELISA and WB.
- Application
- DB, ELISA, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | TP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P04637
- NCBI GenBank
- NM_000546, NP_000537.3
- SwissProt
- P04637
Target
- Target
- Human TP53 / p53
- Antigen Species
- Hu
- Epitope
- A BLAST analysis used to suggest reactivity with this protein from human based on 100% homology for the immunizing sequence.
- Gene Name
- TP53
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-p53 K292 Ac antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 292 of human p53.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl