Detection of Human p53 by Western Blot. Samples: Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody: Rabbit anti-p53 antibody used at the indicated concentrations. Detection: Chemiluminescence with an exposure time of less than 1 minute.
TP53 / p53 Antibody (aa375-C-term)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287179-10
Catalog Number: LS-C287179
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- Description
- P53 antibody LS-C287179 is an unconjugated rabbit polyclonal antibody to human p53 (TP53) (aa375-C-term). Validated for IHC, IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | TP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P04637
- NCBI GenBank
- NM_000546 | NP_000537.3
- SwissProt
- P04637
Target
- Target
- Human TP53 / p53
- Antigen Species
- Human
- Epitope
- Region between residues 375 and the C-terminus (residue 393) of human tumor protein p53 using the numbering given in SwissProt entry P04637 (GeneID 7157).
- Gene Name
- TP53
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 7157 corresponding to amino acids between 375 and the C-Terminus of UniprotID: P04637
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate