Confocal microscopy of human HeLa cells using anti-p53 (BP53-12; FITC). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC MAb. Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic
TP53 / p53 Antibody (aa16-25, clone BP53-12)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$316.00each
Catalog Number: LS-C45758-0.1
Catalog Number: LS-C45758
For bulk orders or custom formulations:
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- Description
- P53 antibody LS-C45758 is an unconjugated mouse monoclonal antibody to p53 (TP53) (aa16-25) from human. It is reactive with human and non-human primates. Validated for ELISA, Flow, ICC, IHC, IP and WB.
- Application
- ELISA, FC, ICC, IHC, IHC-P, IP, WB
- Reactivity
- Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | TP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
- Recommended Dilution: WB
- 1 - 2 µg/ml
- UniProt Number
- P04637
- NCBI GenBank
- NM_000546 | NP_000537.3
- SwissProt
- P04637
Target
- Target
- Human TP53 / p53
- Antigen Species
- Human
- Epitope
- The antibody BP53-12 recognizes defined epitope (aa 16-25) on human p53, a 50 kDa tumor suppressor found in increased amounts in a wide variety of transformed cells; it is frequently mutated or inactivated in many types of cancer.
- Gene Name
- TP53
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- BP53-12
- Isotype
- IgG2a
Immunogen
- Immunogen
- Bacterially expressed full-length wild-type p53
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 15 mM Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C. Do not freeze.
- Recommended Storage Buffer
- PBS