Immunofluorescence staining of TOP2B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TOP2B polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.
TOP2B / Topoisomerase II Beta Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C820568-100
Catalog Number: LS-C820568
For bulk orders or custom formulations:
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- Description
- Topoisomerase II Beta antibody LS-C820568 is an unconjugated rabbit polyclonal antibody to human Topoisomerase II Beta (TOP2B). Validated for ICC and IF.
- Application
- ICC, IF
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DNA topoisomerase II beta | DNA topoisomerase 2-beta | Topo II beta | Antigen MLAA-44 | TOPIIB | Topoisomerase II beta | Topoisomerase IIb | U937 associated antigen | DNA topoisomerase II, 180 kD | TOP2B | Top2beta
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- UniProt Number
- Q02880
- NCBI GenBank
- NM_001068 | NP_001059.2
- SwissProt
- Q02880
Target
- Target
- Human TOP2B / Topoisomerase II Beta
- Antigen Species
- Human
- Epitope
- Human TOP2B
- Gene Name
- TOP2B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived Human TOP2B fragment
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.0, 0.03% Proclin 300
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS