1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
TOP2B / Topoisomerase II Beta Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C800402-100
Catalog Number: LS-C800402
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Topoisomerase II Beta antibody LS-C800402 is an unconjugated rabbit polyclonal antibody to Topoisomerase II Beta (TOP2B) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DNA topoisomerase II beta | DNA topoisomerase 2-beta | Topo II beta | Antigen MLAA-44 | TOPIIB | Topoisomerase II beta | Topoisomerase IIb | U937 associated antigen | DNA topoisomerase II, 180 kD | TOP2B | Top2beta
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- Q02880
- NCBI GenBank
- NM_001068 | NP_001059.2
- SwissProt
- Q02880
Target
- Target
- Human TOP2B / Topoisomerase II Beta
- Antigen Species
- Human
- Epitope
- TOP2B antibody detects endogenous levels of total TOP2B.
- Gene Name
- TOP2B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human TOP2B.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS