Western blot testing of 1) human HepG2, 2) HeLa and 3) mouse NIH3T3 cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.
TOP2A / Topoisomerase II Alpha Antibody (clone TOP2A/1361, Azide-free, BSA-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C761936-100
Catalog Number: LS-C761936
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- Description
- Topoisomerase II Alpha antibody LS-C761936 is an unconjugated mouse monoclonal antibody to human Topoisomerase II Alpha (TOP2A). Validated for Flow, IF, IHC and WB.
- Application
- FC, IF, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- DNA topoisomerase II, 170 kD | TOP2 | TOP2A | DNA gyrase | DNA topoisomerase 2-alpha | TP2A | Topoisomerase IIa
- Recommended Dilution: FC
- 0.5 - 1 µg/10E6 cells
- Recommended Dilution: IF/ICC
- 1 - 2 µg/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 1 - 2 µg/ml
- UniProt Number
- P11388
- NCBI GenBank
- NM_001067 | NP_001058.2
- SwissProt
- P11388
Target
- Target
- Human TOP2A / Topoisomerase II Alpha
- Antigen Species
- Human
- Gene Name
- TOP2A
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- TOP2A/1361
- Isotype
- IgG2b
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 1X PBS
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 2-8°C (with azide) or aliquot and store at -20°C (without azide).
- Recommended Storage Buffer
- 1X PBS