HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A.
TOP2A / Topoisomerase II Alpha Antibody (aa1100-1531, clone 1D2, Carrier-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$522.00each
Catalog Number: LS-C797992-100
Catalog Number: LS-C797992
For bulk orders or custom formulations:
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- Description
- Topoisomerase II Alpha antibody LS-C797992 is an unconjugated mouse monoclonal antibody to human Topoisomerase II Alpha (TOP2A) (aa1100-1531). Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- DNA topoisomerase II, 170 kD | TOP2 | TOP2A | DNA gyrase | DNA topoisomerase 2-alpha | TP2A | Topoisomerase IIa
- Recommended Dilution: IHC
- 1:150
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- P11388
- NCBI GenBank
- NM_001067 | NP_001058.2
- SwissProt
- P11388
Target
- Target
- Human TOP2A / Topoisomerase II Alpha
- Antigen Species
- Human
- Gene Name
- TOP2A
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 1D2
- Isotype
- IgG1
- Origin Species
- Human
Immunogen
- Immunogen
- Human recombinant protein fragment corresponding to amino acids 1100-1531 of human TOP2A(NP_001058) produced in E.coli.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- PBS, pH 7.3, 8% Trehalose
- Preservative
- No
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS