Western blot analysis of extracts of various cell lines, using TNNI3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

TNNI3 / Cardiac Troponin I Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

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$281.00each

Catalog Number: LS-C746728-20

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Quantity

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Overview

Description: Cardiac Troponin I antibody LS-C746728 is an unconjugated rabbit polyclonal antibody to Cardiac Troponin I (TNNI3) from human. It is reactive with human, mouse and rat. Validated for WB.
Application: WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CTnI | CMD1FF | CMD2A | RCM1 | Troponin I, cardiac | Troponin I, cardiac muscle | TNNI3 | Troponin I type 3 (cardiac) | Cardiac troponin I | CMH7
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P19429
NCBI GenBank: NM_000363 | NP_000354.4
SwissProt: P19429

Target

Target: Human TNNI3 / Cardiac Troponin I
Antigen Species: Human
Gene Name: TNNI3

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TNNI3 (NP_000354.4). MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume: 20 Microliter
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: PBS

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