Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
TNFSF14 / LIGHT Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C800446-100
Catalog Number: LS-C800446
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- LIGHT antibody LS-C800446 is an unconjugated rabbit polyclonal antibody to LIGHT (TNFSF14) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD258 antigen | CD258 | Delta transmembrane LIGHT | Herpesvirus entry mediator A | HVEM-L | TR2 | TNFSF14 | HVEML | LIGHT | LTg
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- O43557
- NCBI GenBank
- NM_003807 | NP_003798.2
- SwissProt
- O43557
Target
- Target
- Human TNFSF14 / LIGHT
- Antigen Species
- Human
- Epitope
- TNF14 Antibody detects endogenous levels of TNF14.
- Gene Name
- TNFSF14
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human TNF14.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS