Flow Cytometry analysis of U937 cells using anti-TNF Receptor I antibody. Overlay histogram showing U937 cells stained with anti-TNF Receptor I antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNF Receptor I Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
TNFRSF1A / TNFR1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756388-100
Catalog Number: LS-C756388
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- Description
- TNFR1 antibody LS-C756388 is an unconjugated rabbit polyclonal antibody to TNFR1 (TNFRSF1A) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, ICC, IHC and WB.
- Application
- ELISA, FC, ICC, IHC-F, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD120a | CD120a antigen | MS5 | p60 | TBP1 | TNF-R55 | TNFAR | TNFR1 | p55-R | TNF-R | TNF-R-I | TNF-R1 | TNF-RI | Tnfr | TNFR-I | TNFR1-d2 | TNFR60 | TNFRSF1A | FPF | p55 | Tbpi | TNFR55
- Recommended Dilution: ELISA
- 1 - 5 ug/ml
- Recommended Dilution: FC
- 1 - 3 µg/10E6 cells
- Recommended Dilution: IF/ICC
- 0.5 - 1 µg/ml
- Recommended Dilution: IHC-F
- 0.5 - 1 µg/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 0.1 - 0.5 µg/ml
- UniProt Number
- P19438
- NCBI GenBank
- NM_001065 | NP_001056.1
- SwissProt
- P19438
Target
- Target
- Human TNFRSF1A / TNFR1
- Antigen Species
- Human
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- TNFRSF1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived human TNF Receptor I recombinant protein (Position: I22-T211).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl