Immunofluorescence staining of HVEM in Raji cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human HVEM monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.
TNFRSF14 / CD270 / HVEM Antibody (clone 028, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818981-100
Catalog Number: LS-C818981
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- Description
- HVEM antibody LS-C818981 is an unconjugated rabbit monoclonal antibody to human HVEM (TNFRSF14 / CD270). Validated for ELISA, ICC and IF.
- Application
- ELISA, ICC, IF
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD270 | CD270 antigen | CD40-like protein | Herpesvirus entry mediator | Herpesvirus entry mediator A | HVEM | LIGHTR | Herpes virus entry mediator A | TR2 | ATAR | HVEA | TNFRSF14
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:20 - 1:100
- UniProt Number
- Q92956
- NCBI GenBank
- NM_003820 | NP_003811.2
- SwissProt
- Q92956
Target
- Target
- Human TNFRSF14 / CD270 / HVEM
- Antigen Species
- Human
- Epitope
- Human HVEM
- Gene Name
- TNFRSF14
Antibody
- Host Species
- Rabbit
- Clonality
- Monoclonal
- Clone
- 028
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human HVEM/TNFRSF14/CD270 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS