Western Blot of rabbit anti-ORAI-1 antibody. Marker: Opal Pre-stained ladder Lane 1: MOLT-4 WCL. Lane 2: MDA-MB-435 WCL. Lane 3: Jurkat WCL. Load: 35 µg per lane. Primary antibody: ORAI-1 antibody at 1:1,000 for 3 hrs at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 32 kDa for ORAI-1.
TMEM142A / ORAI1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744634-25
Catalog Number: LS-C744634
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- Description
- ORAI1 antibody LS-C744634 is an unconjugated rabbit polyclonal antibody to human ORAI1 (TMEM142A). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CRACM1 | ORAT1 | ORAI1 | Protein orai-1 | TMEM142A | Transmembrane protein 142A
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q96D31
- NCBI GenBank
- NM_032790 | NP_116179.2
- SwissProt
- Q96D31
Target
- Target
- Human TMEM142A / ORAI1
- Antigen Species
- Human
- Epitope
- The antiserum was further cross-absorbed against MBP by chromatography. Cross-reactivity with ORAI1 from other sources have not been determined.
- Gene Name
- ORAI1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 45 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.01 M Sodium Phosphate|0.15 M NaCl