Rabbit antibody to TLR9 (830-880). IF on human plasmacytoid dendritic cells. Cells were fixed with 2% formaldehyde for 10 minutes at 37?C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to TLR9 (830-880)for 30 min at RT (diluted 20 ug/ml in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI.