Western blot using the affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA.
THRA / THR Alpha Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745311-25
Catalog Number: LS-C745311
For bulk orders or custom formulations:
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- Description
- THR Alpha antibody LS-C745311 is an unconjugated rabbit polyclonal antibody to human THR Alpha (THRA). Validated for ELISA, IF, IHC, IP and WB.
- Application
- ELISA, IF, IHC, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AR7 | C-ERBA-1 | C-erbaalpha | EAR-7.1/EAR-7.2 | ERB-T-1 | ERBA1 | EAR-7 | ERBA-related 7 | HTR alpha 1 | HTR alpha 2 | THRA1 | Thyroid hormone receptor alpha | T3R | Tralpha2 | Triiodothyronine receptor | V-erbA-related protein 7 | Thyroid hormone receptor | Tralpha1 | NR1A1 | THRA3 | C-erbA-alpha | CHNG6 | EAR7 | ERBA | Erba-alpha | THRA | THRA2 | TR alpha | Tralpha
- Recommended Dilution: ELISA
- 1:300000
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IHC
- 1:200
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P10827
- NCBI GenBank
- NM_003250 | NP_003241.2
- SwissProt
- P10827
Target
- Target
- Human THRA / THR Alpha
- Antigen Species
- Human
- Epitope
- This antibody reacts with human THRA protein and recognizes THRA isoform 1. A BLAST analysis was used to suggest cross-reactivity with THRA from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRA from other sources and isoforms may occur but has not been determined.
- Gene Name
- THRA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.89 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl