Anti-THRA Antibody - Western Blot. Western blot of affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kD, indicated by the arrowhead, corresponds to THRA. Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.
THRA / THR Alpha Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C60119-100
Catalog Number: LS-C60119
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- THR Alpha antibody LS-C60119 is an unconjugated rabbit polyclonal antibody to human THR Alpha (THRA). Validated for ELISA, IF, IHC, IP and WB. Cited in 1 publication.
- Application
- ELISA, IF, IHC, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AR7 | C-ERBA-1 | C-erbaalpha | EAR-7.1/EAR-7.2 | ERB-T-1 | ERBA1 | EAR-7 | ERBA-related 7 | HTR alpha 1 | HTR alpha 2 | THRA1 | Thyroid hormone receptor alpha | T3R | Tralpha2 | Triiodothyronine receptor | V-erbA-related protein 7 | Thyroid hormone receptor | Tralpha1 | NR1A1 | THRA3 | C-erbA-alpha | CHNG6 | EAR7 | ERBA | Erba-alpha | THRA | THRA2 | TR alpha | Tralpha
- Recommended Dilution: ELISA
- 1:300000
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IHC
- 1:200
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P10827
- NCBI GenBank
- NM_003250 | NP_003241.2
- SwissProt
- P10827
Target
- Target
- Human THRA / THR Alpha
- Antigen Species
- Human
- Epitope
- This antibody reacts with human THRA protein. A BLAST analysis was used to suggest cross-reactivity with THRA from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRA from other sources has not been determined.
- Gene Name
- THRA
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Persistent COUP-TFII expression underlies the myopathy and impaired muscle regeneration observed in resistance to thyroid hormone-alpha. Paola Aguiari, Yan-Yun Liu, Astgik Petrosyan, Sheue-Yann Cheng, Gregory A Brent, Laura Perin, Anna Milanesi. Scientific reports. 2021 February;11:4601. PubMed: 33633251 PMC: PMC7907286